采用四氯化碳模型研究萝卜叶提取物对大鼠的体外抗氧化及体内肝脏保护活性。
In vitro antioxidant and in vivo hepatoprotective activity of leave extract of Raphanus sativus in rats using CCL4 model.
作者信息
Syed Shariq Naeem, Rizvi Waseem, Kumar Anil, Khan Aijaz Ahmad, Moin Shagufta, Ahsan Akif
机构信息
Department of Pharmacology, MAMC, Dehli University, New Delhi, India 110002.
Department of Pharmacology, JNMC, Aligarh Muslim University, Aligarh (UP), India 202002.
出版信息
Afr J Tradit Complement Altern Med. 2014 Apr 3;11(3):102-6. doi: 10.4314/ajtcam.v11i3.15. eCollection 2014.
BACKGROUND
Raphanus sativus is reported to have a variety of biological activities. This work screened the hepato-protective and antioxidant activity of ethanol (ERS), and aqueous (ARS), extracts of leaves of Raphanus sativus in Carbon tetrachloride (CCl4), model in rats.
MATERIAL AND METHODS
The extracts were subjected to antioxidant tests (Total reducing power and Total phenolic content), and preliminary phytochemical screening. A pilot study was done on 100 and 300 mg/kg extracts, form which 300 mg was chosen for further experiments. The albino rats (200-250 grams), were divided into 5 groups of 6 animals each (n=6). There were three control groups comprising of normal control (normal saline -1ml/kg), negative control group (CCl4 1ml/kg in olive oil in a ratio of 1:1 v/v), and positive control group (Silymarin 50mg/kg). The Test drugs were given in a dose of 300 mg/kg for both ERS and ARS extract for 7 days. Biochemical parameters (AST, ALT, Alkaline phosphatase, Total Bilirubin), histo-pathological examination of liver and in vivo antioxidant tests [CAT, GSH and MDA] were done.
RESULTS
The phytochemical study showed the presence of flavanoids, terpenoids, alkaloids, saponins and sterols. A dose dependent increase in the oxidative potential was observed in both the extracts with total phenolic content 70.1 and 44.4 GAE/g extract for ERS and ARS respectively. ERS 300mg/kg showed a significant (p<0.001) increase in levels of AST, ALT and alkaline phosphatase as compared to negative control (percentage hepatoprotection =45.3%) while ARS 300 mg/kg (p<.01) group showed 30% hepatoprotection. The GSH (p<0.001) and CAT (p<0.05) in ERS and ARS were significantly increased while MDA levels were decreased (P< 0.01), as compared negative control. The findings were confirmed histo-pathological examination.
CONCLUSION
The ethanol and aqueous extract of Raphanus sativus have partial hepatoprotection against CCl4 toxicity.
背景
据报道，萝卜具有多种生物活性。本研究筛选了萝卜叶乙醇提取物（ERS）和水提取物（ARS）对大鼠四氯化碳（CCl4）模型的肝脏保护和抗氧化活性。
材料与方法
对提取物进行抗氧化试验（总还原力和总酚含量）以及初步的植物化学筛选。对100和300mg/kg提取物进行了初步研究，从中选择300mg进行进一步实验。将白化大鼠（200-250克）分为5组，每组6只动物（n = 6）。有三个对照组，包括正常对照组（生理盐水-1ml/kg）、阴性对照组（CCl4 1ml/kg与橄榄油按1:1 v/v比例混合）和阳性对照组（水飞蓟宾50mg/kg）。ERS和ARS提取物均以300mg/kg的剂量给药7天。进行了生化参数（谷草转氨酶、谷丙转氨酶、碱性磷酸酶、总胆红素）、肝脏组织病理学检查以及体内抗氧化试验[过氧化氢酶、谷胱甘肽和丙二醛]。
结果
植物化学研究表明存在黄酮类、萜类、生物碱、皂苷和甾醇。两种提取物均观察到氧化电位呈剂量依赖性增加，ERS和ARS的总酚含量分别为70.1和44.4 GAE/g提取物。与阴性对照组相比，ERS 300mg/kg组的谷草转氨酶、谷丙转氨酶和碱性磷酸酶水平显著升高（p<0.001）（肝脏保护百分比=45.3%），而ARS 300mg/kg组（p<0.01）显示出30%的肝脏保护作用。与阴性对照组相比，ERS和ARS中的谷胱甘肽（p<0.001）和过氧化氢酶（p<0.05）显著增加，而丙二醛水平降低（P<0.01）。组织病理学检查证实了这些发现。
结论
萝卜的乙醇提取物和水提取物对CCl4毒性具有部分肝脏保护作用。
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