不依赖培养的阴道微生物群分析：阴道阿托波菌与细菌性阴道病未被认识的关联。
Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis.
作者信息
Verstraelen Hans, Verhelst Rita, Claeys Geert, Temmerman Marleen, Vaneechoutte Mario
机构信息
Department of Obstetrics and Gynaecology, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium.
出版信息
Am J Obstet Gynecol. 2004 Oct;191(4):1130-2. doi: 10.1016/j.ajog.2004.04.013.
Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.
尽管细菌性阴道病的发病机制仍不清楚，但一些微生物，如阴道加德纳菌，被认为是革兰氏染色或培养中的标志物。使用16S rDNA克隆和对总细菌群落进行测序对阴道微生物群进行非培养分析，结果显示革兰氏阳性的阴道阿托波菌是阴道菌群紊乱时的主要菌种。
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