Zühlke Sebastian, Dünnbier Uwe, Heberer Thomas
Institute of Food Chemistry, Technical University Berlin, Sekr TIB 4/3-1, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
J Chromatogr A. 2004 Oct 1;1050(2):201-9. doi: 10.1016/j.chroma.2004.08.051.
A new analytical method applying in situ derivatization was developed to enable the extraction of polar drug metabolites from water samples by solid-phase extraction (SPE). An additional derivatization by silylation was used to enhance the sensitivity of analyte detection by gas chromatography-mass spectrometry (GC-MS). Thus, the two metabolites 1,5-di-methyl-1,2-dehydro-3-pyrazolone (DP) and 4-(2-methylethyl)-1,5-dimethyl-1,2-dehydro-3-pyrazolone (PDP), postulated for the degradation of phenazone and propyphenazone, were identified and detected up to the microg/L level in raw and drinking water samples from public water supply.
开发了一种采用原位衍生化的新分析方法，以通过固相萃取（SPE）从水样中提取极性药物代谢物。使用硅烷化进行额外衍生化，以提高气相色谱 - 质谱联用（GC-MS）对分析物检测的灵敏度。因此，在公共供水的原水和饮用水样品中，鉴定并检测到了推测为非那宗和丙非那宗降解产物的两种代谢物1,5 - 二甲基 - 1,2 - 脱氢 - 3 - 吡唑啉酮（DP）和4 -（2 - 甲基乙基）- 1,5 - 二甲基 - 1,2 - 脱氢 - 3 - 吡唑啉酮（PDP），检测限可达微克/升水平。
