Kros J M, van Eden C G, Vissers C J, Mulder A H, van der Kwast T H
Academic Hospital Rotterdam-Dijkzigt, Department of Clinical Pathology, The Netherlands.
Cancer. 1992 Apr 1;69(7):1791-8. doi: 10.1002/1097-0142(19920401)69:7<1791::aid-cncr2820690722>3.0.co;2-z.
In a retrospective study of 85 cases, the prognostic value of DNA flow cytometry in oligodendrogliomas was evaluated. Paraffin-embedded material was processed for flow cytometry, and the survival rates of the patients with DNA diploid, aneuploid, and tetraploid tumors were compared using analysis of variance. In addition, the mitotic index was correlated with the results of flow cytometry. Finally, the results of flow cytometry, histopathologic grading, and counting mitoses were tested for dependency. Thirty-one percent of the tumors were diploid, 39% were tetraploid, and 31% were aneuploid. The results of the DNA flow cytometry did not correlate with the survival times (P = 0.798) or with tumor degree. In contrast, the number of mitoses (P less than 0.05), and the grades of the grading system of Smith (P less than 0.003) had relevance for the prognosis. No correlation between flow cytometry, histopathologic grading, and mitotic index was found. It is concluded that flow cytometry has no value in predicting the biologic behavior of oligodendrogliomas, whereas the number of mitoses is a valuable prognostic parameter and thus is considered to be incorporated into the grading system for oligodendrogliomas.
在一项对85例病例的回顾性研究中，评估了DNA流式细胞术在少突胶质细胞瘤中的预后价值。对石蜡包埋材料进行流式细胞术处理，采用方差分析比较DNA二倍体、非整倍体和四倍体肿瘤患者的生存率。此外，将有丝分裂指数与流式细胞术结果进行关联。最后，检验流式细胞术结果、组织病理学分级和有丝分裂计数之间的相关性。31%的肿瘤为二倍体，39%为四倍体，31%为非整倍体。DNA流式细胞术结果与生存时间（P = 0.798）或肿瘤分级均无相关性。相比之下，有丝分裂数（P < 0.05）以及史密斯分级系统的分级（P < 0.003）与预后相关。未发现流式细胞术、组织病理学分级和有丝分裂指数之间存在相关性。得出的结论是，流式细胞术在预测少突胶质细胞瘤的生物学行为方面没有价值，而有丝分裂数是一个有价值的预后参数，因此应纳入少突胶质细胞瘤的分级系统。
