Jimenez O, Timms A, Quirke P, McLaughlin J E
Department of Histopathology, Royal Free Hospital School of Medicine, London.
Neuropathol Appl Neurobiol. 1989 Jul-Aug;15(4):331-8. doi: 10.1111/j.1365-2990.1989.tb01233.x.
The DNA ploidy of a series of 78 gliomas has been estimated by flow cytometry using cell suspensions prepared from paraffin embedded material. Apart from two oligodendrogliomas the tumours were all astrocytomas. Forty-nine (63%) tumours were found to have a diploid DNA distribution and 29 (37%) an aneuploid DNA distribution. The two oligodendrogliomas were both DNA aneuploid. No correlation was found between DNA ploidy and histological grade or DNA ploidy and survival. Both ploidy groups appear to have responded equally to therapy. In this study the factors associated with a short survival were advancing age and the presence of vascular endothelial proliferation, while the DNA ploidy and the cytological features do not appear as useful in predicting survival when examining biopsy material.
利用从石蜡包埋材料制备的细胞悬液，通过流式细胞术对78例胶质瘤的DNA倍性进行了评估。除了2例少突胶质细胞瘤外，其余肿瘤均为星形细胞瘤。发现49例（63%）肿瘤具有二倍体DNA分布，29例（37%）具有非整倍体DNA分布。这2例少突胶质细胞瘤均为DNA非整倍体。未发现DNA倍性与组织学分级或DNA倍性与生存率之间存在相关性。两个倍性组对治疗的反应似乎相同。在本研究中，与生存期短相关的因素是年龄增长和血管内皮细胞增殖的存在，而在检查活检材料时，DNA倍性和细胞学特征在预测生存率方面似乎并无用处。
