A23187对分离的小鼠肝细胞中甲基乙二醛和丙酮酸生成葡萄糖的影响。
The effect of A23187 on glucose production from methylglyoxal and pyruvate in isolated murine hepatocytes.
作者信息
Kalapos M P, Riba P, Garzó T, Antoni F, Mandl J
机构信息
Semmelweis University, Medical School, First Institute of Biochemistry, Budapest, Hungary.
出版信息
Int J Biochem. 1992 Mar;24(3):411-4. doi: 10.1016/0020-711x(92)90032-v.
PMID:1551455
Abstract
- A23187 increased the glucose production from methylglyoxal in isolated hepatocytes, and maximal stimulation was obtained at 10(-6) M. The effect of A23187 was dependent on the presence of Ca2+. 2. Glucose production from pyruvate (less than 1 mM) in isolated hepatocytes was stimulated by A23187 in the presence of 2.5 mM Ca2+ and was depressed at pyruvate concentrations above 1 mM. Both the virtual Km and the virtual Vmax of glucose production from pyruvate were decreased by A23187.
摘要
- A23187增加了分离肝细胞中甲基乙二醛的葡萄糖生成量，在10⁻⁶ M时获得最大刺激。A23187的作用依赖于Ca²⁺的存在。2. 在2.5 mM Ca²⁺存在下，A23187刺激分离肝细胞中丙酮酸（小于1 mM）的葡萄糖生成，而在丙酮酸浓度高于1 mM时则受到抑制。A23187降低了丙酮酸生成葡萄糖的表观Km和表观Vmax。
Zotero 插件