Kalapos M P, Riba P, Garzo T, Mandl J
First Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Experientia. 1996 Aug 15;52(8):827-30. doi: 10.1007/BF01923997.
Acetol and methylglyoxal are intermediates of the intrahepatic metabolism of acetone leading to pyruvate formation. In hepatocytes prepared from fasted streptozotocin-induced diabetic mice, net glucose production could be measured from methylglyoxal but not from acetone or acetol. Insulin increased glucose formation from methylglyoxal in a concentration-dependent manner, whereas it was ineffective when pyruvate was used as substrate. Drug oxidation, as evidenced by p-aminophenol formation from aniline, was enhanced by methylglyoxal, and insulin proved to be stimulatory in this case as well. It is concluded that insulin might be involved in the regulation of glucose formation from methylglyoxal, but its mode of action is not yet clear.
丙酮醇和甲基乙二醛是丙酮肝内代谢生成丙酮酸的中间产物。在由禁食的链脲佐菌素诱导的糖尿病小鼠制备的肝细胞中，可以检测到由甲基乙二醛产生的净葡萄糖生成，但丙酮或丙酮醇则不能。胰岛素以浓度依赖的方式增加了由甲基乙二醛生成的葡萄糖，而当丙酮酸用作底物时，胰岛素则无效。药物氧化，如由苯胺生成对氨基酚所证明的，被甲基乙二醛增强，并且在这种情况下胰岛素也被证明具有刺激作用。得出的结论是，胰岛素可能参与了由甲基乙二醛生成葡萄糖的调节，但其作用方式尚不清楚。
