鹑鸡肠球菌BM4174的vanC基因序列，该基因编码一种对万古霉素耐药性必需的D-丙氨酸：D-丙氨酸连接酶相关蛋白。
Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase-related protein necessary for vancomycin resistance.
作者信息
Dutka-Malen S, Molinas C, Arthur M, Courvalin P
机构信息
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
出版信息
Gene. 1992 Mar 1;112(1):53-8. doi: 10.1016/0378-1119(92)90302-6.
The amplification product obtained with DNA from vancomycin-resistant (VmR) Enterococcus gallinarum BM4174 and a pair of degenerate oligodeoxyribonucleotides that correspond to conserved amino acid (aa) motifs in Escherichia coli D-alanine (D-Ala):D-Ala ligases and in En. faecium VmR protein (VanA) was used as a probe to clone the vanC gene of that strain. The vanC product, with a calculated Mr of 37,504, exhibits 29 to 38% aa identity with VanA and E. coli ligases. Insertional inactivation of vanC led to Vm sensitivity of BM4174 suggesting that the gene may encode a D-Ala:D-Ala ligase of altered specificity.
从耐万古霉素（VmR）鸡肠球菌BM4174中提取的DNA，与一对简并寡脱氧核糖核苷酸一起扩增得到的产物用作探针，该对寡脱氧核糖核苷酸对应于大肠杆菌D-丙氨酸（D-Ala）：D-Ala连接酶和屎肠球菌VmR蛋白（VanA）中的保守氨基酸（aa）基序，用于克隆该菌株的vanC基因。vanC产物的计算分子量为37,504，与VanA和大肠杆菌连接酶的氨基酸同一性为29%至38%。vanC的插入失活导致BM4174对Vm敏感，这表明该基因可能编码一种特异性改变的D-Ala：D-Ala连接酶。
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