Rodríguez Rodríguez E, Henríquez Sánchez P, López Blanco F, Díaz Romero C, Serra Majem L
Departamento de Química Analítica, Nutrición y Bromatología, Universidad de La Laguna, Santa Cruz de Tenerife.
Nutr Hosp. 2004 Sep-Oct;19(5):263-8.
Serum concentrations of Na, K, Ca, Mg, Fe, Cu, Zn, Se, Mn and P were determined in apparently health individuals representing of the population of the Canary Islands. Multivariate analysis was applied on the data matrix in order to differentiate the individuals according several criteria such as gender, age, island and province of residence, smoking and drinking habits and physical exercise.
395 serum samples (187 men and 208 women) were analyzed mean age of 38.4 +/- 20.0 years. Individuals data about age, gender, weight, height, alcohol consumption, smoking habits and physical exercise were recorded using standardized questionnaires. The determination of minerals was carried out by flame emission spectrometry (Na and K) and atomic absorption spectrometry with flame air/acetylene (Ca, Mg, Fe, Cu, Zn), hybride generation (Se) and graphite furnace (Mn). The P was determined by a colorimetric method.
The sex and age of individuals influenced on the serum concentrations of some minerals, Cu and Fe, and P and Se, respectively. The island of residence influenced the mean concentrations of the most the minerals analysed. The smoking and drinking habits do not seem to influence the mean contents of the minerals in an important manner. Physical exercise had significant influence on the P, Cu and Mn concentrations in serum. The water for consumption influenced on the serum concentrations of the electrolytes and Ca and Mg, but it did not affect the concentrations of the trace elements. Applying discriminant analysis the individuals lower 18 years were reasonably well differentiated (89% of the individuals correctly classified) from the rest of individuals. A tendency for differentiation of individuals according to the island of residence was also observed.
A low differentiation of the individuals according to the sex, province or island or residence and habits or life style was observed after application of multivariate analysis techniques. However, the adults were reasonably differentiated from the children and adolescent, and the inhabitants of Lanzarote and La Palma tend to separate from the rest of the individuals of their province.
测定加那利群岛具有代表性的健康人群血清中钠、钾、钙、镁、铁、铜、锌、硒、锰和磷的浓度。对数据矩阵进行多变量分析，以便根据性别、年龄、居住岛屿和省份、吸烟和饮酒习惯以及体育锻炼等多种标准对个体进行区分。
分析了395份血清样本（187名男性和208名女性），平均年龄为38.4±20.0岁。使用标准化问卷记录个体有关年龄、性别、体重、身高、饮酒量、吸烟习惯和体育锻炼的数据。矿物质的测定采用火焰发射光谱法（钠和钾）以及火焰空气/乙炔原子吸收光谱法（钙、镁、铁、铜、锌）、氢化物发生法（硒）和石墨炉法（锰）。磷采用比色法测定。
个体的性别和年龄分别影响某些矿物质的血清浓度，铜和铁、磷和硒。居住岛屿影响所分析的大多数矿物质的平均浓度。吸烟和饮酒习惯似乎未对矿物质的平均含量产生重要影响。体育锻炼对血清中的磷、铜和锰浓度有显著影响。饮用水影响电解质以及钙和镁的血清浓度，但不影响微量元素的浓度。应用判别分析，18岁以下个体与其他个体得到了较好区分（89%的个体正确分类）。还观察到根据居住岛屿对个体进行区分的趋势。
应用多变量分析技术后，未观察到根据性别、省份或岛屿、居住情况以及习惯或生活方式对个体进行的低区分度。然而，成年人与儿童和青少年得到了合理区分，兰萨罗特岛和拉帕尔马岛的居民倾向于与该省其他个体区分开来。
