Blake Thomas A, Ouyang Zheng, Wiseman Justin M, Takáts Zoltán, Guymon Andrew J, Kothari Sameer, Cooks R Graham
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Anal Chem. 2004 Nov 1;76(21):6293-305. doi: 10.1021/ac048981b.
A preparative mass spectrometer for microarray fabrication is reported. The instrument includes an atmospheric pressure ionization source, a linear ion trap mass analyzer, an ion collection surface positioning system, and a surface loading chamber with independent vacuum pumping. It was designed for the production of protein arrays using the ion soft-landing technique to collect ions on a surface after separation by mass/charge ratio. Small microarrays have been prepared by isolating and soft landing individual protein or peptide ions after electrospray ionization of mixtures. The composition and purity of the separated materials has been confirmed using independent external mass spectrometric analysis of rinse solutions of the collected spots, either by the new method of electrosonic spray ionization MS or by nanospray ionization MS. The ability to retain bioactivity in the mass-selected and collected biomolecules has been demonstrated in particular cases. The reported instrument has also been characterized as an analytical mass spectrometer.
报道了一种用于微阵列制造的制备型质谱仪。该仪器包括大气压电离源、线性离子阱质量分析器、离子收集表面定位系统以及具有独立真空泵抽气的表面加载腔室。它被设计用于利用离子软着陆技术生产蛋白质阵列，以便在通过质荷比分离后将离子收集在表面上。通过对混合物进行电喷雾电离后分离并软着陆单个蛋白质或肽离子，制备了小型微阵列。已通过对收集斑点的冲洗溶液进行独立的外部质谱分析，采用电超声喷雾电离质谱新方法或纳米喷雾电离质谱，确认了分离材料的组成和纯度。在特定情况下已证明了在质量选择和收集的生物分子中保留生物活性的能力。所报道的仪器也已被表征为分析型质谱仪。
