在单分子水平上对蛋白质进行成像。
Imaging proteins at the single-molecule level.
作者信息
Longchamp Jean-Nicolas, Rauschenbach Stephan, Abb Sabine, Escher Conrad, Latychevskaia Tatiana, Kern Klaus, Fink Hans-Werner
机构信息
Physics Department of the University of Zurich, CH-8057 Zurich, Switzerland;
Max Planck Institute for Solid State Research, DE-70569 Stuttgart, Germany.
出版信息
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1474-1479. doi: 10.1073/pnas.1614519114. Epub 2017 Jan 13.
Abstract
Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process.
摘要
对单个蛋白质进行成像一直是推动自然科学各个领域发展的长期目标，例如结构生物学、生物物理学和分子纳米技术。特别是，揭示单个蛋白质的不同构象至关重要。在这里，我们展示了通过低能电子全息术对单个蛋白质和蛋白质复合物进行成像。通过软着陆电喷雾离子束沉积制备了超清洁独立石墨烯上的单个蛋白质和蛋白质复合物样品，该方法允许进行化学和构象特异性选择以及温和沉积。低能电子不会引起辐射损伤，这使得能够获得单个蛋白质（细胞色素C和牛血清白蛋白）以及蛋白质复合物（血红蛋白）的亚纳米分辨率图像，这些图像不是平均过程的结果。
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