Saucan L, Palade G E
Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093.
Hepatology. 1992 Apr;15(4):714-21. doi: 10.1002/hep.1840150427.
We carried out a comparative investigation on the effects of colchicine (25 mumoles/100 gm body wt) on the intracellular transport, processing and discharge by secretion or proteolytic processing of a membrane protein (i.e., the polymeric IgA receptor) and a secretory protein (i.e., albumin) in rat hepatocytes. The results obtained indicated the following: (a) the transport and processing of polymeric IgA receptor is strongly inhibited and delayed, but the appearance of secretory component in the bile is not arrested; (b) polymeric IgA receptor reaches the sinusoidal plasmalemma in colchicine-treated specimens, as it does in controls; (c) albumin discharge into the plasma is strongly inhibited and markedly delayed in colchicine-treated as compared with control animals; (d) the reverse applies for albumin secretion in the bile, which is increased by a large factor; (e) newly synthesized albumin secreted directly from hepatocytes in control and in colchicine-treated animals is the major source of bile albumin; and (f) colchicine affects in different ways the polymeric IgA receptor and albumin arrival at the sinusoidal front and especially at the biliary front of the hepatocyte.
我们对秋水仙碱（25微摩尔/100克体重）对大鼠肝细胞中一种膜蛋白（即聚合免疫球蛋白A受体）和一种分泌蛋白（即白蛋白）的细胞内运输、加工以及通过分泌或蛋白水解加工进行释放的影响进行了比较研究。获得的结果表明如下：（a）聚合免疫球蛋白A受体的运输和加工受到强烈抑制并延迟，但胆汁中分泌成分的出现并未停止；（b）在秋水仙碱处理的标本中，聚合免疫球蛋白A受体到达窦状质膜，与对照标本情况相同；（c）与对照动物相比，秋水仙碱处理的动物中白蛋白释放到血浆中受到强烈抑制且明显延迟；（d）胆汁中白蛋白分泌的情况则相反，其分泌量大幅增加；（e）对照动物和秋水仙碱处理动物中从肝细胞直接分泌的新合成白蛋白是胆汁白蛋白的主要来源；（f）秋水仙碱以不同方式影响聚合免疫球蛋白A受体和白蛋白到达肝细胞窦状前沿，尤其是胆管前沿。
