Fujikawa M, Yamashita N, Yamazaki K, Sugiyama E, Suzuki H, Hamazaki T
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
Immunology. 1992 Feb;75(2):330-5.
Recently, many investigators have studied the effects of eicosapentaenoic acid (EPA)-rich fish oil on immune function and immune disease. However, effects of dietary supplementation of fish oil or EPA on the immune system are still unclear. In the present study, the effects of EPA on antigen presentation were investigated. We have used antigen-specific helper T-cell clones that proliferate in the presence of antigen [keyhole limpet haemocyanin (KLH)] and spleen cells as antigen-presenting cells (APC). Mice were divided into two groups and fed an experimental diet or a control diet for 4 weeks ad libitum. In mice fed the experimental diet, the arachidonic acid (AA) content of spleen cells was decreased and that of EPA and docosapentaenoic acid was increased markedly compared to those of the control diet. Dietary enrichment with EPA inhibited the ability of accessory cells to present antigen to murine helper T-cell clones. This effect was observed for two distinct helper T-cell clones, Th1 and Th2. We also examined the effects of EPA-TG emulsion on APC function. The direct addition of EPA-TG emulsion to a T-cell proliferation assay system suppressed APC function. The inhibition was proportional to the concentration of EPA-TG emulsion. Pretreatment of splenocytes with EPA-TG emulsion resulted in inhibition of APC function. Inhibition of antigen presentation by dietary supplementation with EPA might depress immune reactivity.
最近，许多研究人员研究了富含二十碳五烯酸（EPA）的鱼油对免疫功能和免疫疾病的影响。然而，膳食补充鱼油或EPA对免疫系统的影响仍不明确。在本研究中，我们研究了EPA对抗抗原呈递的影响。我们使用了在抗原[匙孔血蓝蛋白（KLH）]存在下增殖的抗原特异性辅助性T细胞克隆，并将脾细胞作为抗原呈递细胞（APC）。将小鼠分为两组，随意喂食实验性饮食或对照饮食4周。与对照饮食组相比，喂食实验性饮食的小鼠脾细胞中花生四烯酸（AA）含量降低，而EPA和二十二碳五烯酸含量显著增加。膳食中富含EPA会抑制辅助细胞向小鼠辅助性T细胞克隆呈递抗原的能力。在两种不同的辅助性T细胞克隆Th1和Th2中均观察到了这种效应。我们还研究了EPA-TG乳剂对APC功能的影响。将EPA-TG乳剂直接添加到T细胞增殖检测系统中会抑制APC功能。这种抑制作用与EPA-TG乳剂的浓度成正比。用EPA-TG乳剂预处理脾细胞会导致APC功能受到抑制。膳食补充EPA对抗原呈递的抑制可能会降低免疫反应性。
