[Metabolic activity of peripheral blood neutrophils in women with ovarian tumours].

OBJECTIVES AND DESIGN

Neutrophils play important role in first line defence an they release soluble growth and chemotactic factors and guide the recruitment of non-specific immune effector cells. We estimated the activity and ability to reduce of nitroblue tetrazolium (NBT) by peripheral blood neutrophils in women with benign or malignant ovarian tumours.

MATERIALS AND METHODS

The study was performed on 43 women between the ages of 19 and 72 with ovarian tumours. Cystadenoma serosum was diagnosed in 19 women, Cystadenocarcinoma serosum in 9, and teratoma adultum in 15 women. Venous blood samples were obtained prior to the operation. The control group consisted of 30 women their age range was from 22 to 60 years. In peripheral blood samples from both groups; there leukocyte counts and total neutrophils were determined. Metabolic activity was investigated by nitroblue tetrazolium reduction spontaneous (NBTsp) test, and stimulation by latexs (NBTst) test.

RESULTS

In women with ovarian tumours, the index of spontaneous NBT reduction turned out to be significantly higher than that in the control group (p<0.0001). In the group studied, the index of latex-stimulated NBT reduction was significantly lower than that in the control group (p<0.0001).

CONCLUSIONS

Intensity oxidation-reduction changes of neutrophils in reduction NBT in women with ovarian tumours were observed.