[Phagocytosis and NBT test and phosphatase activity of peripheral blood neutrophils in patients with untreated duodenal ulcer].

In 21 patients with active, endoscopically confirmed untreated duodenal ulcer and in a control group of 20 persons, the studies were carried out of peripheral blood neutrophils which included: 1) test with the assessment of neutrophil phagocytic activity against live bacterial cells of the Staphylococcus aureus Oxford 209P standard strain and against latex particles, 2) test of nitroblue tetrazolium reduction (NBT test) by neutrophils, 3) evaluation of alkaline phosphatase activity in the neutrophils by a cytoenzymatic technique. In the group of patients as compared with the control group a decrease was observed of neutrophil phagocytic activity against live bacterial cells, and absence of similar differences was noted when latex particles were the stimulating factor. In the studied group an increase of the value of the index of spontaneous NBT reduction by neutrophils was observed as well as a decrease of the value of the index of latex-stimulated reduction of this dye. Besides that, higher alkaline phosphatase activity was observed in the neutrophils in patients as compared to the activity of this enzyme in the neutrophils of healthy persons. It may be supposed that in patients with duodenal ulcer, peripheral blood neutrophils reveal changes in the function of membranous structures of these cells, and disturbances of redox processes evaluated in the test with NBT.