Chamberland H, Lafontaine J G
Biology Department, Faculty of Science and Engineering, Laval University, Quebec, Canada.
Histochem J. 1992 Jan;24(1):1-8. doi: 10.1007/BF01043280.
Sugars have been demonstrated in animal cell nuclei, but only a few studies have mentioned their presence in plant cell nuclei. In this study L-fucose residues were localized at the ultrastructural level, using Ulex europeaus agglutinin I lectin, during the early stages of germination of Pisum sativum and in mature root tip cells. This sugar was present after 1 h of germination, and its concentration was found to vary during 3 to 6 h inhibition; after 72h of inhibition its concentration had more than doubled. Furthermore, labelling was particularly abundant in the nucleolus, nucleolus-associated bodies and dense nuclear bodies. The possibility that some of the L-fucose residues are associated with proteins is discussed.
糖类已在动物细胞核中得到证实，但仅有少数研究提及它们在植物细胞核中的存在。在本研究中，利用欧洲荆豆凝集素I，在豌豆萌发早期以及成熟根尖细胞中，在超微结构水平上定位了L-岩藻糖残基。这种糖在萌发1小时后就已存在，并且发现在3至6小时的抑制过程中其浓度有所变化；抑制72小时后，其浓度增加了一倍多。此外，在核仁、核仁相关体和致密核体中标记特别丰富。本文还讨论了一些L-岩藻糖残基与蛋白质相关的可能性。
