Department of Cardiology, The First Affiliated Hospital, Shantou University Medical College, Shantou, China.
Laboratory of Molecular Cardiology, The First Affiliated Hospital, Shantou University Medical College, Shantou, China.
J Cell Mol Med. 2022 Jan;26(2):306-311. doi: 10.1111/jcmm.17079. Epub 2021 Dec 14.
Endothelial progenitor cells (EPCs), which are precursors of endothelial cells (ECs), have the capacity to circulate, proliferate and differentiate into mature ECs. EPCs are primarily identified by the uptake of 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labelled acetylated low-density lipoprotein (Dil-acLDL) and the binding of fluorescein-isothiocyanate (FITC)-conjugated Ulex europaeus agglutinin lectin (FITC-UEA-I). However, the cytoplasm and nucleus are usually stained by FITC-UEA-I via a typical method to double-stain late EPCs. It is necessary to explore a new method to improve the quality of fluorescence photomicrographs of late EPCs stained with FITC-UEA-I. Here, we described an updated protocol for double-staining late EPCs with Dil-acLDL and FITC-UEA-I, with the cells more optimally stained with FITC-UEA-I.
内皮祖细胞（EPCs）是内皮细胞（ECs）的前体细胞，具有循环、增殖和分化为成熟 ECs 的能力。EPCs 主要通过摄取 1,1-二辛基-3,3,3,3-四甲基吲哚羰花青标记的乙酰化低密度脂蛋白（Dil-acLDL）和荧光素异硫氰酸酯（FITC）-缀合的欧洲菘蓝凝集素（FITC-UEA-I）来识别。然而，通常通过 FITC-UEA-I 的典型方法对细胞质和细胞核进行双重染色，以对晚期 EPCs 进行双重染色。有必要探索一种新的方法来提高用 FITC-UEA-I 染色的晚期 EPCs 的荧光显微照片的质量。在这里，我们描述了一种用 Dil-acLDL 和 FITC-UEA-I 对晚期 EPCs 进行双重染色的更新方案，该方案使 FITC-UEA-I 对细胞的染色效果更加优化。
