Seve A P, Hubert J, Bouvier D, Masson C, Geraud G, Bouteille M
J Submicrosc Cytol. 1984 Oct;16(4):631-41.
Nuclear glycoconjugates were detected in situ by two lectins--Concanavalin A and Wheat germ agglutinin--on tissue sections embedded in the hydrophilic resin glycol methacrylate. These lectins were conjugated with fluorescein isothiocyanate for fluorescence microscopy, and labelled with ferritin for electron microscopy. The ovarian follicle of the lizard Lacerta vivipara was chosen for this study, because it enables four types of nuclei, with different ultrastructures and physiology to be observed on the same section. In this material, all the nucleoli were found to contain a high concentration of receptors for both lectins. The distribution of the receptors located in the chromatin and nucleoli was observed to vary within the same type of nucleus, depending on the lectin used. Some of our results suggest that increased labelling by Wheat germ agglutinin might depend on increased production of ribosomal precursors.
通过两种凝集素——刀豆球蛋白A和麦胚凝集素，在包埋于亲水性树脂甲基丙烯酸乙二醇酯中的组织切片上对核糖缀合物进行原位检测。这些凝集素与异硫氰酸荧光素结合用于荧光显微镜观察，并用铁蛋白标记用于电子显微镜观察。本研究选用胎生蜥蜴丽斑麻蜥的卵巢卵泡，因为在同一切片上可以观察到四种具有不同超微结构和生理学特征的细胞核。在这种材料中，发现所有核仁都含有高浓度的两种凝集素受体。观察到位于染色质和核仁中的受体分布在同一类型的细胞核内会因所用凝集素的不同而有所变化。我们的一些结果表明，麦胚凝集素标记增加可能取决于核糖体前体产量的增加。
