De Smedt S C, Remaut K, Lucas B, Braeckmans K, Sanders N N, Demeester J
Laboratory of General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
Adv Drug Deliv Rev. 2005 Jan 2;57(1):191-210. doi: 10.1016/j.addr.2004.06.003.
Advanced light microscopy (ALM) has been intensively employed by biophysicists to reveal cellular mechanisms. As described in this review, ALM clearly has potential to enhance our understanding of the mechanisms that affect macromolecular therapeutics or nanoscopic drug vectors in biological environments. However, while in recent years confocal microscopy and related techniques became rather routinely used in drug delivery it remains challenging to extract reliable information on the biophysical behaviour of drug delivery systems from ALM measurements. This review discusses studies in which confocal imaging, fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and fluorescence energy transfer were employed to reveal biophysical properties of DNA and DNA containing nanoparticles in extra- and intracellular media.
高级光学显微镜（ALM）已被生物物理学家广泛用于揭示细胞机制。如本综述所述，ALM显然有潜力增进我们对生物环境中影响大分子治疗药物或纳米药物载体机制的理解。然而，尽管近年来共聚焦显微镜及相关技术在药物递送中已相当常规地使用，但从ALM测量中提取关于药物递送系统生物物理行为的可靠信息仍然具有挑战性。本综述讨论了利用共聚焦成像、光漂白后荧光恢复（FRAP）、荧光相关光谱（FCS）和荧光能量转移来揭示细胞外和细胞内介质中DNA及含DNA纳米颗粒生物物理特性的研究。
