Takamatsu Hiroshi, Takeya Ryu, Naito Seiji, Sumimoto Hideki
Institute for Materials Chemistry and Engineering, Kyushu University, 6-1 Kasugakoen, Kasuga, Fukuoka 816-8580, Japan.
J Biomech. 2005 Jan;38(1):117-24. doi: 10.1016/j.jbiomech.2004.03.011.
In this study, we identify the extent of deformation that causes cell lysis using a simple technique where a drop of cell suspension is compressed by two flat plates. The viability of human prostatic adenocarcinoma PC-3 cells in solutions of various concentrations of NaCl is determined as a function of the gap size between the plates. The viability declines with decreasing gap size in the following order: 700 mM >150 mM >75 mM NaCl. This is considered to be due to the difference in cell size, which is caused by the osmotic volume change before deformation; cell diameter becomes smaller in a solution of higher NaCl concentration, which appears to increase the survival ratio in a given gap size. The deformation-induced decrease in cell viability is correlated with the cell surface strain, which is dependent on the increase in surface area, irrespective of NaCl concentration. In addition, the treatment of cells with cytochalasin D results in the disappearance of cortical actin filaments and a marked drop in the viability, indicating that cell lysis is closely related to the deformation of the cytoskeleton.
在本研究中，我们使用一种简单的技术来确定导致细胞裂解的变形程度，该技术是通过两块平板压缩一滴细胞悬液。测定了人前列腺腺癌PC - 3细胞在不同浓度NaCl溶液中的活力，并将其作为平板间间隙大小的函数。细胞活力随间隙大小减小按以下顺序下降：700 mM > 150 mM > 75 mM NaCl。这被认为是由于细胞大小的差异，这是由变形前的渗透体积变化引起的；在较高NaCl浓度的溶液中细胞直径变小，这似乎在给定间隙大小下提高了存活率。变形引起的细胞活力下降与细胞表面应变相关，细胞表面应变取决于表面积的增加，与NaCl浓度无关。此外，用细胞松弛素D处理细胞会导致皮质肌动蛋白丝消失和活力显著下降，表明细胞裂解与细胞骨架的变形密切相关。
