通过气相色谱法对微升生化样品中的十二烷基硫酸钠进行定量分析。

Quantification of sodium dodecyl sulfate in microliter biochemical samples by gas chromatography.

作者信息

Campbell Reika, Winkler Martin A, Wu Huaiqin

机构信息

Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA.

出版信息

Anal Biochem. 2004 Dec 1;335(1):98-102. doi: 10.1016/j.ab.2004.08.026.

DOI:10.1016/j.ab.2004.08.026

PMID:15519576

Abstract

A novel gas chromatography (GC) method has been developed to accurately quantitate sodium dodecyl sulfate (SDS) in aqueous biochemical samples. This method is based on the quantitative conversion of SDS to 1-dodecanol in the GC injection port at elevated temperature, and the thermal degradation product 1-dodecanol was analyzed to determine SDS concentration. It was found that the addition of guanidinium chloride (GnHCl) to SDS samples (via direct dilution with GnHCl/MeOH solution) is necessary to ensure accurate quantitation. The presence of GnHCl enables quantitative conversion of SDS to 1-dodecanol, improves sensitivity, and virtually eliminates interference from proteins and other chemicals commonly present in biochemical samples. The method features direct analysis of diluted SDS samples, is free from interference, and is capable of quantifying less than 1 ng SDS in biochemical samples. It is also suitable for samples with limited volume, with as little as 1 microl sample being sufficient for quantitation.

摘要

已开发出一种新型气相色谱（GC）方法，用于准确定量分析水性生化样品中的十二烷基硫酸钠（SDS）。该方法基于在高温下将SDS在GC进样口中定量转化为1-十二醇，并通过分析热降解产物1-十二醇来确定SDS浓度。研究发现，向SDS样品中添加氯化胍（GnHCl）（通过用GnHCl/甲醇溶液直接稀释）对于确保准确定量是必要的。GnHCl的存在能够使SDS定量转化为1-十二醇，提高灵敏度，并几乎消除生化样品中常见的蛋白质和其他化学物质的干扰。该方法的特点是直接分析稀释后的SDS样品，无干扰，能够定量生化样品中少于1 ng的SDS。它也适用于体积有限的样品，仅1微升样品就足以进行定量分析。