Dunselman G A, Willebrand D, Evers J L
Department of Obstetrics and Gynaecology, Academic Hospital Maastricht, The Netherlands.
Hum Reprod. 1992 Jan;7(1):73-5. doi: 10.1093/oxfordjournals.humrep.a137563.
Oestrogen receptors (ER) and progesterone receptors (PR) were measured in the rabbit model of endometriosis in eutopic and ectopic endometrium of pregnant animals (n = 7) and controls (n = 7). Immunostaining of cryostat sections of ectopic and eutopic endometrium was performed using monoclonal antibodies against ER and PR. Levels of ER and PR were 'evaluated' in a semi-quantitative manner using a modified histoscore. The ER and PR content in stromal and glandular cells was not different in eutopic and ectopic endometrium in either pregnant or non-pregnant control animals. There was a significant difference between the PR content of the glandular epithelium of pregnant animals and controls for both eutopic (P less than 0.02) and ectopic (P less than 0.001) endometrium. The disappearance of glandular PR and the persistence of stromal PR suggest that the function of the glandular endometrium is mediated by the paracrine and autocrine action of stromal cells. The decidual cells are likely to produce substances that may be of importance in embryo implantation and early pregnancy.
在怀孕动物（n = 7）和对照组（n = 7）的子宫内膜异位症兔模型中，对在位和异位子宫内膜中的雌激素受体（ER）和孕激素受体（PR）进行了检测。使用抗ER和PR的单克隆抗体对异位和在位子宫内膜的冰冻切片进行免疫染色。采用改良的组织学评分半定量评估ER和PR水平。在怀孕或未怀孕的对照动物中，在位和异位子宫内膜的基质细胞和腺细胞中的ER和PR含量没有差异。怀孕动物与对照组在位（P<0.02）和异位（P<0.001）子宫内膜的腺上皮PR含量存在显著差异。腺PR的消失和基质PR的持续存在表明，腺子宫内膜的功能是由基质细胞的旁分泌和自分泌作用介导的。蜕膜细胞可能产生对胚胎着床和早期妊娠很重要的物质。
