恶性疟原虫生命周期中转录本和蛋白质水平的全局分析。
Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle.
作者信息
Le Roch Karine G, Johnson Jeffrey R, Florens Laurence, Zhou Yingyao, Santrosyan Andrey, Grainger Munira, Yan S Frank, Williamson Kim C, Holder Anthony A, Carucci Daniel J, Yates John R, Winzeler Elizabeth A
机构信息
Department of Cell Biology ICND202, the Scripps Research Institute, La Jolla, California 92037, USA.
出版信息
Genome Res. 2004 Nov;14(11):2308-18. doi: 10.1101/gr.2523904.
Abstract
To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.
摘要
为了研究转录后调控在疟原虫（恶性疟原虫）蛋白质表达调控中的作用，我们比较了该寄生虫生命周期七个不同阶段的mRNA转录本和蛋白质丰度水平。在这些阶段观察到mRNA和蛋白质丰度之间存在适度的高度正相关；最常见的差异是mRNA和蛋白质积累之间的延迟。我们鉴定了潜在的转录后调控基因，并观察到功能相关基因家族具有相似的mRNA和蛋白质积累模式。
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