Herba Ewa, Pojda-Wilczek Dorota, Plech Agata R, Pojda Stefan M, Szkilnik Ryszard
Department of Ophthalmology and Eye Division, Silesian Medical University, Katowice, Municipal Hospital No. 1, Zeromskiego 7, PL 41-902 Bytom, Poland.
Pol J Pharmacol. 2004 Jul-Aug;56(4):415-9.
The female adult white Wistar rats were given tap water (control) or 50 ppm of methylmercury chloride (MMC) ad libitum throughout their pregnancies. Newborn rats drank mother's milk during the first 21 days after delivery and then only tap water. The study was carried out on three-month old offsprings of white Wistar rats. The flash visual evoked potentials (FVEP) were recorded before and after injecting of 10 microl 0.9% saline, 50 or 100 nmols of dopamine (DA) into the lateral brain ventricle by method used before in our laboratory. The amplitude of the first deep negative (N(1)) peak significantly increased to 109-114% after both doses of DA in the control group and to 138-139% in mercury-treated animals. The amplitude of the next positive (P(1)) wave decreased to 94% and 86% in the control group after 50 and 100 nmols of DA, respectively. In Hg-treated group after 50 nmols of DA, the value dropped down to 91%, but increased to 109% after 100 nmols of DA. The increasing of DeltaN(1)P(1) was observed in the control group to 112% after 50 nmols and to 109% after 100 nmols of DA and in Hg-exposed rats, respectively, to 127% and to 129%. The described changes were statistically significant (p < 0.05). The N(1) and P(1) latencies were prolonged in the control group after both doses of DA. In Hg-treated group, the prolongation of N(1) latency was recorded, while the P(1) latency was not changed. We concluded that prenatal Hg intoxication disturbed the effect of DA on FVEP.
成年雌性Wistar白鼠在整个孕期随意饮用自来水（对照组）或50 ppm的氯化甲基汞（MMC）。新生大鼠在出生后的前21天饮用母乳，之后只饮用自来水。该研究针对Wistar白鼠三个月大的后代进行。通过我们实验室之前使用的方法，在向侧脑室注射10微升0.9%生理盐水、50或100纳摩尔多巴胺（DA）之前和之后记录闪光视觉诱发电位（FVEP）。在对照组中，两种剂量的DA注射后，第一个深负波（N(1)）峰的幅度显著增加至109 - 114%，而在汞处理的动物中增加至138 - 139%。在对照组中，分别注射50和100纳摩尔DA后，下一个正波（P(1)）的幅度分别降至94%和86%。在汞处理组中，注射50纳摩尔DA后，该值降至91%，但注射100纳摩尔DA后增加至109%。在对照组中，注射50纳摩尔DA后DeltaN(1)P(1)增加至112%，注射100纳摩尔DA后增加至109%，在汞暴露大鼠中分别增加至127%和129%。上述变化具有统计学意义（p < 0.05）。在对照组中，两种剂量的DA注射后N(1)和P(1)潜伏期均延长。在汞处理组中，记录到N(1)潜伏期延长，而P(1)潜伏期未改变。我们得出结论，产前汞中毒扰乱了多巴胺对闪光视觉诱发电位的影响。
