Abgrall J F, el-Kassar N, Berthou C, Renard I, Cauvin J M, Le Pailleur A, Autrand C, Sensebe L, Guern G, Zilliken P
Department of Hematology, Hôpital A. Morvan, Brest University, School of Medicine, France.
Int J Cell Cloning. 1992 Jan;10(1):28-32. doi: 10.1002/stem.5530100106.
In vitro megakaryocyte colony formation from the bone marrow of patients with acute idiopathic thrombocytopenic purpura (ITP) or chronic ITP was compared using a plasma clot system. The number of megakaryocyte colony-forming units (CFU-Meg) was significantly higher (p less than 0.05) in acute ITP compared to chronic ITP (54.3 +/- 68.4 vs. 12.9 +/- 15.3/10(5) nonadherent mononuclear cells, mean +/- SD), and significantly lower (p less than 0.05) in chronic ITP compared to controls (12.9 +/- 15.3 vs. 22.8 +/- 15.9). A significant correlation was observed between platelet recovery 7 and 30 days after culture, and the number of CFU-Meg (r = 0.49 and 0.45, respectively, p less than 0.05). An inverse correlation was observed between platelet count at the time of culture and the number of Megs per colony (r = -0.48, p less than 0.05). These results indicated a difference between acute and chronic ITP in the ability to promote in vitro Meg colony formation and may suppose a different immune mechanism for thrombocytopenia in these two disorders.
使用血浆凝块系统比较了急性特发性血小板减少性紫癜（ITP）或慢性ITP患者骨髓的体外巨核细胞集落形成情况。与慢性ITP相比，急性ITP中巨核细胞集落形成单位（CFU-Meg）的数量显著更高（p<0.05）（54.3±68.4对12.9±15.3/10⁵非贴壁单核细胞，均值±标准差），而与对照组相比，慢性ITP中的CFU-Meg数量显著更低（p<0.05）（12.9±15.3对22.8±15.9）。培养后7天和30天的血小板恢复情况与CFU-Meg数量之间存在显著相关性（r分别为0.49和0.45，p<0.05）。培养时的血小板计数与每个集落中的巨核细胞数量呈负相关（r=-0.48，p<0.05）。这些结果表明急性和慢性ITP在促进体外巨核细胞集落形成的能力上存在差异，并且可能提示这两种疾病中血小板减少的免疫机制不同。
