用于从卡介苗（牛分枝杆菌）中纯化基因组DNA组分的离子交换色谱法

Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin.

作者信息

Jing Wang, Shuhan Sun, Zhenlin Hu, Fengjuan Zhou, Yiling Ling

机构信息

Department of Pathophysiology, Hebei Medical University, Shijiazhuang, China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 25;811(2):103-7. doi: 10.1016/j.jchromb.2004.06.035.

DOI:10.1016/j.jchromb.2004.06.035

PMID:15522708

Abstract

The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA.

摘要

本研究的目的是提供通过离子交换色谱法从卡介苗（BCG-DNA）中纯化基因组DNA组分的实用策略。开发了一种多步骤方法来纯化BCG-DNA。该过程包括超声处理、加热、胰蛋白酶消化、离子交换色谱、凝胶过滤色谱和冻干。经过离子交换色谱后，BCG-DNA得到了高度纯化，并具有强大的生物学效应。所描述的方法高效且具有良好的重现性。此外，这是首次报道的纯化BCG-DNA的色谱方法。