Seki Masaaki, Honda Ikuro, Fujita Isao, Yano Ikuya, Yamamoto Saburo, Koyama Akira
Japan BCG Laboratory, Research of Product, 3-1-5 Matsuyama, Kiyose City, Tokyo 204-0022, Japan.
Vaccine. 2009 Mar 10;27(11):1710-6. doi: 10.1016/j.vaccine.2009.01.034. Epub 2009 Feb 4.
To investigate the molecular characteristics of bacillus Calmette-Guérin (BCG) vaccines, the complete genomic sequence of Mycobacterium bovis BCG Tokyo 172 was determined, and the results were compared with those for BCG Pasteur and other M. tuberculosis complex. The genome of BCG Tokyo had a length of 4,371,711bp and contained 4033 genes, including 3950 genes coding for proteins (CDS). There were 18 regions of difference (showing differences of more than 20bp), 20 insertion or deletion (ins/del) mutations of less than 20bp, and 68 SNPs between the two BCG substrains. These findings are useful for better understanding of the genetic differences in BCG substrains due to in vitro evolution of BCG.
为研究卡介苗（BCG）疫苗的分子特征，测定了牛分枝杆菌卡介苗东京172株的全基因组序列，并将结果与卡介苗巴斯德株及其他结核分枝杆菌复合群进行比较。卡介苗东京株的基因组长度为4,371,711bp，包含4033个基因，其中包括3950个编码蛋白质的基因（CDS）。两种卡介苗亚株之间存在18个差异区域（差异超过20bp）、20个小于20bp的插入或缺失（ins/del）突变以及68个单核苷酸多态性（SNP）。这些发现有助于更好地理解卡介苗体外传代导致的亚株间遗传差异。
