Aranishi Futoshi, Okimoto Takane
Department of Biological and Environmental Sciences, Miyazaki University, Miyazaki 889-2192, Japan.
J Appl Genet. 2004;45(4):435-43.
We developed random amplified polymorphic DNA (RAPD) analysis for the assessment of the genetic relationship between cultured populations of the Pacific oyster Crassostrea gigas Thunberg in Hiroshima and Goseong, the largest oyster farming areas in Japan and Korea, respectively. Of 25 arbitrary primers comprising decamer nucleotides of random sequences, polymerase chain reaction amplifications with 5 different primers gave reproducible electrophoretic patterns. A total of 49 RAPD markers were clearly identified for the Hiroshima and Goseong populations, and 46 markers were polymorphic presenting mean polymorphism rates of the respective populations at 92.29% and 93.32%. Pairwise genetic distances of each 20 individuals from these populations served to produce a UPGMA dendrogram. The dendrogram comprised two main clusters, one of which was a nested cluster including all individuals of the Hiroshima population along with 12 individuals of the Goseong population, and the other cluster included the remaining individuals of the Goseong population. Results indicate that RAPD markers are useful for the assessment of the genetic relationships between populations of the Pacific oyster and further that a significant portion of oysters imported from Korea could be genetically related to the Hiroshima population.
我们开发了随机扩增多态性DNA（RAPD）分析方法，以评估分别位于日本广岛和韩国固城这两个日本和韩国最大牡蛎养殖区的养殖太平洋牡蛎群体之间的遗传关系。在由随机序列的十聚体核苷酸组成的25种任意引物中，用5种不同引物进行的聚合酶链反应扩增产生了可重复的电泳图谱。广岛和固城群体共明确鉴定出49个RAPD标记，其中46个标记具有多态性，各自群体的平均多态性率分别为92.29%和93.32%。来自这些群体的每组20个个体的成对遗传距离用于生成一个UPGMA树状图。该树状图包括两个主要聚类，其中一个是嵌套聚类，包括广岛群体的所有个体以及固城群体的12个个体，另一个聚类包括固城群体的其余个体。结果表明，RAPD标记可用于评估太平洋牡蛎群体之间的遗传关系，进一步表明从韩国进口的很大一部分牡蛎可能与广岛群体存在遗传关系。
