Chen Y, Wang W, Yang Y, Su B, Zhang Y, Xiong L, He Z, Shu C, Yang D
Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences.
Yi Chuan Xue Bao. 1997 Oct;24(5):410-6.
Random amplified polymorphic DNA(RAPD) has been used in analyzing 13 winter worm, summer grasses (Cordyceps sinensis [Berk.], Sacc.) from 5 areas in Qingzang plateau. For 19 arbitrary primers, each individual showed about 65 RAPD markers. The genetic distances among those 13 winter worm, summer grasses were correlated to their geographic distances. Molecular phylogenetic tree constructed by UPGMA and NJ suggested that 13 winter worm, summer grasses could be divided into 3 clusters: east cluster, north cluster and middle cluster, in consistence with their origins and genetic divergence among different clusters. Furthermore, our results implied that the RAPD markers of winter worm, summer grasses showed a geographic specificity and could be a better genetic marker in studying genetic diversity and phylogeny of winter worm, summer grasses.
随机扩增多态性DNA（RAPD）已被用于分析来自青藏高原5个地区的13个冬虫夏草样本。对于19个随机引物，每个个体显示出约65个RAPD标记。这13个冬虫夏草样本之间的遗传距离与其地理距离相关。通过UPGMA和NJ构建的分子系统发育树表明，13个冬虫夏草样本可分为3个聚类：东部聚类、北部聚类和中部聚类，这与它们的来源以及不同聚类之间的遗传差异一致。此外，我们的结果表明，冬虫夏草的RAPD标记具有地理特异性，并且在研究冬虫夏草的遗传多样性和系统发育方面可能是一个更好的遗传标记。
