Zhang Ling-ling, Wei Wei, Yan Shang-xue, Hu Xiang-yang, Sun Wu-yi
Institute of Clinical Pharmacology, Anhui Medical University, Hefei, China.
Acta Pharmacol Sin. 2004 Nov;25(11):1495-501.
To investigate the therapeutic effect of the glucosides of Cheanomeles speciosa (GCS) on the collagen-induced arthritis (CIA) in mice.
Mice were divided randomly into six groups, including normal, CIA, CIA+GCS (60, 120, and 240 mg/kg) and CIA plus glucosides of Tripterygium wilfordii (GTW) groups. CIA model was based on mice. The effect of GCS in CIA mice was measured by paw-swelling, arthritis scores, and histopathological assessment of synovium. Indices of thymus and spleens were measured. Thymocytes and splenocytes proliferation, activity of interleukin-1 (IL-1), and interleukin-2 (IL-2) were assayed by MTT and [(3)H]TdR method. The level of anti-collagen type II (CII) antibody in serum and prostaglandin E (PGE) in ankle were assayed by ELISA and ultraviolet spectrophotometer method, respectively.
The onset of paw-swelling was on d 24 after injection of emulsion. The peak of secondary inflammation appeared on d 36 and then declined after d 40. GCS and GTW significantly reduced paw-swelling and arthritis scores, reduced the increase of spleen indices of CIA mice, suppressed the ConA or LPS-induced thymocyte or spleen cell proliferation, and the production of IL-1 and IL-2 in CIA mice. GCS reduced the level of anti-CII antibody and PGE. Histological pathology analysis demonstrated that the synovium of CIA mice was hyperplastic, pannus was formed, and inflammatory cells infiltrated into synovium. The pathological changes were significantly reduced by GCS.
GCS had anti-inflammatory effect on CIA mice, which might be related to the modification of the abnormal immunological function of CIA mice.
探讨木瓜苷（GCS）对小鼠胶原诱导性关节炎（CIA）的治疗作用。
将小鼠随机分为六组，包括正常组、CIA组、CIA+GCS（60、120和240 mg/kg）组以及CIA加雷公藤多苷（GTW）组。以小鼠建立CIA模型。通过足肿胀、关节炎评分及滑膜组织病理学评估来测定GCS对CIA小鼠的作用。测量胸腺和脾脏指数。采用MTT法和[³H]TdR法检测胸腺细胞和脾细胞增殖、白细胞介素-1（IL-1）和白细胞介素-2（IL-2）活性。分别采用ELISA法和紫外分光光度计法检测血清中抗Ⅱ型胶原（CII）抗体水平及踝关节中前列腺素E（PGE）水平。
注射乳剂后第24天出现足肿胀。二次炎症高峰出现在第36天，之后在第40天后下降。GCS和GTW显著减轻足肿胀和关节炎评分，降低CIA小鼠脾脏指数的升高，抑制CIA小鼠中ConA或LPS诱导的胸腺细胞或脾细胞增殖以及IL-1和IL-2的产生。GCS降低抗CII抗体和PGE水平。组织病理学分析表明，CIA小鼠滑膜增生，形成血管翳，炎性细胞浸润滑膜。GCS可显著减轻这些病理变化。
GCS对CIA小鼠具有抗炎作用，这可能与改善CIA小鼠异常免疫功能有关。
