Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immunopharmacology of Education Ministry, Meishan Road, Hefei 230032, Anhui Province, China.
J Ethnopharmacol. 2011 Jan 27;133(2):511-6. doi: 10.1016/j.jep.2010.10.022. Epub 2010 Oct 19.
To investigate the expression of β-arrestins in fibroblast-like synoviocytes (FLS) from collagen-induced arthritis (CIA) rats and the effect of total glucosides of paeony (TGP).
TGP and glucosides of tripterygium wilfordii (GTW) were intragastriclly administrated to collagen-induced arthritis (CIA) rats after immunization. The secondary inflammatory reaction was evaluated by hind paw swelling, polyarthritis index and histopathological changes. Antibodies to type II collagen (CII) were determined by enzyme-linked immunosorbent assay (ELISA). Synoviocyte proliferations were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl (MTT) assay. The expression of β-arrestins in synoviocytes from CIA rats was measured by western blot.
The administration of TGP (25, 50, 100 mg/kg) depressed hind paw swelling and decreased the arthritis scores of CIA rats. TGP improved the pathologic manifestations of CIA. Serum anti-CII antibodies level increased significantly in CIA rats, while TGP had no effect on it. Fibroblast-like synoviocytes (FLS) proliferation was inhibited by TGP (50, 100 mg/kg). On d14, d28 after immunization, β-arrestins expression greatly up-regulated in synoviocytes from CIA rats and then returned to baseline levels on d42 after immunization. TGP (50, 100 mg/kg) significantly reduced the expression of β-arrestins.
An inflammatory process in vivo induces an up-regulation of β-arrestins in synoviocytes from CIA rats while TGP can inhibit this change, which might be one of the important mechanisms for TGP to produce a marked therapeutic effect on RA.
研究β-arrestins 在胶原诱导关节炎（CIA）大鼠成纤维样滑膜细胞（FLS）中的表达及白芍总苷（TGP）的作用。
免疫后，白芍总苷（TGP）和雷公藤多苷（GTW）灌胃胶原诱导关节炎（CIA）大鼠。通过后爪肿胀、多发性关节炎指数和组织病理学变化评估二次炎症反应。酶联免疫吸附试验（ELISA）测定 II 型胶原（CII）抗体。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基（MTT）测定滑膜细胞增殖。用 Western blot 测定 CIA 大鼠滑膜细胞中β-arrestins 的表达。
TGP（25、50、100mg/kg）给药可抑制后爪肿胀，降低 CIA 大鼠关节炎评分。TGP 改善 CIA 的病理表现。CIA 大鼠血清抗 CII 抗体水平显著升高，而 TGP 对此无影响。TGP（50、100mg/kg）抑制成纤维样滑膜细胞（FLS）增殖。在免疫后第 14、28 天，CIA 大鼠滑膜细胞中β-arrestins 表达显著上调，然后在免疫后第 42 天恢复至基线水平。TGP（50、100mg/kg）显著降低β-arrestins 的表达。
体内炎症过程诱导 CIA 大鼠滑膜细胞中β-arrestins 的上调，而 TGP 可抑制这种变化，这可能是 TGP 对 RA 产生显著治疗作用的重要机制之一。
