Dai Jun, Li Jian, Bos Erik, Porcionatto Marimelia, Premont Richard T, Bourgoin Sylvain, Peters Peter J, Hsu Victor W
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
Dev Cell. 2004 Nov;7(5):771-6. doi: 10.1016/j.devcel.2004.10.002.
Cargo sorting that promotes the transport of cargo proteins from a membrane compartment has been predicted to be unlikely in the endocytic recycling pathways. We now show that ACAP1 binds specifically and directly to recycling cargo proteins. Reducing this interaction for TfR inhibits its recycling. Moreover, ACAP1 binds to two distinct phenylalanine-based sequences in the cytoplasmic domain of TfR that function as recycling sorting signals to promote its transport from the recycling endosome. Taken together, these findings indicate that ACAP1 promotes cargo sorting by recognizing recycling sorting signals.
促进货物蛋白从膜区室运输的货物分选，据预测在内吞再循环途径中不太可能发生。我们现在表明，ACAP1特异性且直接地与再循环货物蛋白结合。减少TfR的这种相互作用会抑制其再循环。此外，ACAP1与TfR细胞质结构域中两个不同的基于苯丙氨酸的序列结合，这些序列作为再循环分选信号，促进其从再循环内体的运输。综上所述，这些发现表明ACAP1通过识别再循环分选信号促进货物分选。
