Li Jian, Peters Peter J, Bai Ming, Dai Jun, Bos Erik, Kirchhausen Tomas, Kandror Konstantin V, Hsu Victor W
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Boston, MA 02115, USA.
J Cell Biol. 2007 Jul 30;178(3):453-64. doi: 10.1083/jcb.200608033.
Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings, stimulation-dependent recycling of integrin that is critical for cell migration and insulin-stimulated recycling of glucose transporter type 4 (Glut4), which is required for glucose homeostasis. These findings not only advance a basic understanding of an early mechanistic step in endocytic recycling but also shed key mechanistic insights into major physiological events for which this transport plays a critical role.
衣被蛋白是否在胞吞再循环中发挥广泛作用仍不清楚。我们发现，ACAP1是一种针对ADP-核糖基化因子（ARF）6的GTP酶激活蛋白（GAP），它是一种新型网格蛋白衣被复合物的一部分，该复合物在两个关键生理环境中受ARF6调节以进行胞吞再循环，即对细胞迁移至关重要的整合素刺激依赖性再循环，以及葡萄糖稳态所需的胰岛素刺激的4型葡萄糖转运蛋白（Glut4）再循环。这些发现不仅推进了对胞吞再循环早期机制步骤的基本理解，还为这一转运起关键作用的主要生理事件提供了关键的机制见解。
