Itoh Tomohiro, Itoh Yuko, Mizutani Mineo, Fujishiro Katsuhisa, Furuichi Yukio, Komiya Takashi, Hibasami Hiroshige
Imuraya Confectionary Co. Ltd., Takachaya 7-1-1, Tsu, Mie 514-8530, Japan.
J Nutr Sci Vitaminol (Tokyo). 2004 Aug;50(4):295-9. doi: 10.3177/jnsv.50.295.
Treatment of human stomach cancer KATO III cells with hot-water extracts from adzuki beans led to their growth inhibition as well as apoptosis induction. There are morphological changes in the cultured cells treated with the extracts, by which DNA fragmentation characteristic of apoptosis was actualized both concentration- and time-dependently. In contrast, N-acetyl-L-cysteine suppressed such DNA fragmentation, implying that the extracts from adzuki beans might exert antitumorigenicity via active oxygen-induced apoptosis. In order to verify this hypothesis in animal experiment, the 40% ethanol fraction of hot-water extracts was examined for its preventive effect against benzo(a)pyrene-induced tumorigenesis in the forestomach of A/J mice, given as drinking water containing the above fraction at 0.5-2.0% levels. Consequently, forestomach cancer has turned out to be reduced by 36-62% in tumor weight relative to the control. These results suggest that the fraction of hot-water adzuki extracts may serve as a nutrapharmaceutical or functional food available for cancer prevention.
用赤豆热水提取物处理人胃癌KATO III细胞，可导致其生长抑制以及诱导细胞凋亡。用提取物处理的培养细胞会出现形态变化，通过这种变化，凋亡特有的DNA片段化呈浓度和时间依赖性地得以实现。相比之下，N-乙酰-L-半胱氨酸可抑制这种DNA片段化，这意味着赤豆提取物可能通过活性氧诱导的细胞凋亡发挥抗肿瘤作用。为了在动物实验中验证这一假设，研究了热水提取物的40%乙醇馏分对A/J小鼠前胃中苯并(a)芘诱导的肿瘤发生的预防作用，将上述馏分以0.5-2.0%的水平添加到饮用水中给予小鼠。结果发现，相对于对照组，前胃癌的肿瘤重量减少了36-62%。这些结果表明，赤豆热水提取物馏分可作为一种用于癌症预防的营养药物或功能性食品。
