Leu Yann-Lii, Hwang Tsong-Long, Kuo Ping-Chung, Liou Kun-Pei, Huang Bow-Shin, Chen Guo-Feng
Graduate Institute of Natural Products, Chang Gung University, Taoyuan 333, Taiwan.
Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan.
Int J Mol Sci. 2012;13(8):9754-9768. doi: 10.3390/ijms13089754. Epub 2012 Aug 6.
The seeds of Vigna genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of V. vexillata demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of V. vexillata. One new sterol (1) and two new isoflavones (2,3) were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23), abscisic acid (25), and quercetin (40) displayed the most significant inhibition of superoxide anion generation and elastase release.
豇豆属植物的种子是重要的食物资源，并且已经有许多关于其生物活性的报道。在我们的初步生物测定中，毛豇豆甲醇提取物的氯仿层表现出显著的抗炎生物活性。因此，本研究旨在纯化和鉴定毛豇豆的抗炎成分。首次从天然来源报道了一种新的甾醇（1）和两种新的异黄酮（2,3），并通过光谱和质谱分析确定了它们的化学结构。此外，通过将其物理和光谱数据与文献报道的数据进行比较，鉴定出37种已知化合物。在分离出的化合物中，大豆苷元（23）、脱落酸（25）和槲皮素（40）对超氧阴离子生成和弹性蛋白酶释放的抑制作用最为显著。
