Azuine M A, Kayal J J, Bhide S V
Carcinogenesis Division, Tata Memorial Centre, Parel, Bombay, India.
J Cancer Res Clin Oncol. 1992;118(6):447-52. doi: 10.1007/BF01629428.
Turmeric (Curcuma longa Linn.) has been shown to inhibit chemical carcinogenesis. In this study, we compared the chemopreventive efficacy of an aqueous turmeric extract (AqTE) and its constituents, curcumin-free aqueous turmeric extract (CFAqTE) and curcumin, using the Salmonella typhimurium mutagenicity assay and the bone marrow micronucleus test in female Swiss mice. AqTE exhibited antimutagenic activity against direct-acting mutagens, 4-nitro-O-phenylenediamine and 1-methyl-3-nitro-1-nitrosoguanidine, in strains TA 98 and TA 100 respectively. Both AqTE and CFAqTE inhibited the mutagenicity of benzo [alpha]pyrene in the two strains in the presence of Aroclor-1254-induced rat liver homogenate. The inhibition in both studies was dose-dependent. Administration of AqTE, CFAqTE and curcumin at a dose of 3 mg/animal 18 h prior to i.p. benzo [alpha]pyrene injection (250 mg/kg) significantly inhibited bone marrow micronuclei formation in female Swiss mice by 43%, 76%, and 65% respectively. Furthermore, the incidence and multiplicity of forestomach tumours induced by benzo [alpha]pyrene (1 mg/animal, twice weekly, p.o. for 4 weeks) in female Swiss mice were significantly inhibited by AqTE, CFAqTE and curcumin given 2 weeks before, during and after the carcinogen treatment. These data indicate that the protection against genomic damage by turmeric extract and its components tested could be necessary for some aspects of its cancer chemoprevention.
姜黄(Curcuma longa Linn.)已被证明具有抑制化学致癌作用。在本研究中,我们使用鼠伤寒沙门氏菌致突变性试验和雌性瑞士小鼠骨髓微核试验,比较了姜黄水性提取物(AqTE)及其成分无姜黄素的姜黄水性提取物(CFAqTE)和姜黄素的化学预防效果。AqTE对直接作用的诱变剂4-硝基-O-苯二胺和1-甲基-3-硝基-1-亚硝基胍分别在TA 98和TA 100菌株中表现出抗诱变活性。在存在Aroclor - 1254诱导的大鼠肝匀浆的情况下,AqTE和CFAqTE均抑制了两种菌株中苯并[a]芘的诱变性。两项研究中的抑制作用均呈剂量依赖性。在腹腔注射苯并[a]芘(250 mg/kg)前18小时,以3 mg/动物的剂量给予AqTE、CFAqTE和姜黄素,可分别显著抑制雌性瑞士小鼠骨髓微核形成43%、76%和65%。此外,在致癌物处理前、处理期间和处理后2周给予AqTE、CFAqTE和姜黄素,可显著抑制苯并[a]芘(1 mg/动物,每周两次,口服,共4周)诱导的雌性瑞士小鼠前胃肿瘤的发生率和多发性。这些数据表明,姜黄提取物及其测试成分对基因组损伤的保护作用可能是其癌症化学预防某些方面所必需的。