Ovchinnikova Tatiana V, Aleshina Galina M, Balandin Sergey V, Krasnosdembskaya Anna D, Markelov Mikhail L, Frolova Elena I, Leonova Yulia F, Tagaev Andrey A, Krasnodembsky Eugeny G, Kokryakov Vladimir N
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str. 16/10, 117997 Moscow, Russia.
FEBS Lett. 2004 Nov 5;577(1-2):209-14. doi: 10.1016/j.febslet.2004.10.012.
Two novel 21-residue antimicrobial peptides, arenicin-1 and arenicin-2, exhibiting activity against Gram-positive and Gram-negative bacteria and fungi, were purified from coelomocytes of marine polychaeta Arenicola marina (lugworm) by preparative gel electrophoresis and RP-HPLC. Molecular masses (2758.3 and 2772.3 Da) and complete amino acid sequences (RWCVYAYVRVRGVLVRYRRCW and RWCVYAYVRIRGVLVRYRRCW) were determined for each isoform. Each arenicin has one disulfide bond (Cys3-Cys20). The total RNA was isolated from the lugworm coelomocytes, RT-PCR and cloning were performed, and cDNA was sequenced. A 202-residue preproarenicin contains a putative signal peptide (25 amino acids) and a long prodomain. Arenicins have no structure similarity to any previously identified antimicrobial peptides.
从海栖多毛纲动物沙蠋（海蚯蚓）的体腔细胞中，通过制备凝胶电泳和反相高效液相色谱法纯化出了两种新型的21个氨基酸残基的抗菌肽，即沙蠋抗菌肽-1和沙蠋抗菌肽-2，它们对革兰氏阳性菌、革兰氏阴性菌和真菌均具有活性。测定了每种异构体的分子量（分别为2758.3和2772.3道尔顿）以及完整的氨基酸序列（分别为RWCVYAYVRVRGVLVRYRRCW和RWCVYAYVRIRGVLVRYRRCW）。每种沙蠋抗菌肽都有一个二硫键（Cys3-Cys20）。从沙蠋体腔细胞中分离出总RNA，进行逆转录聚合酶链反应（RT-PCR）和克隆，并对cDNA进行测序。一个202个氨基酸残基的前原沙蠋抗菌肽包含一个推定的信号肽（25个氨基酸）和一个长的前结构域。沙蠋抗菌肽与任何先前鉴定的抗菌肽均无结构相似性。
