Callaway Anton S, George Carol G, Lommel Steven A
Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695-7616, USA.
Virology. 2004 Dec 5;330(1):186-95. doi: 10.1016/j.virol.2004.09.037.
Red clover necrotic mosaic virus (RCNMV; genus Dianthovirus) and Turnip rosette virus (TRoV; genus Sobemovirus) are taxonomically and ecologically distinct plant viruses. In addition, the two genera differ in the role of coat protein (CP) in cell-to-cell movement. However, both are small icosahedral viruses requiring CP for systemic movement in the host vasculature. Here, we show that the TRoV CP gene is capable of facilitating the vascular movement of a Dianthovirus. Substitution of the RCNMV CP gene with the TRoV CP gene permits movement of the resulting chimeric virus to non-inoculated leaves. RCNMV lacking a CP gene or containing a non-translatable TRoV CP gene do not move systemically. This report introduces the molecular characterization of TRoV and describes the unprecedented complementation of systemic movement function by intergenic complete substitution of a plant virus CP gene.
红三叶草坏死花叶病毒（RCNMV；石竹病毒属）和芜菁莲座病毒（TRoV；南方菜豆花叶病毒属）在分类学和生态学上是不同的植物病毒。此外，这两个属在外壳蛋白（CP）在细胞间移动中的作用方面也有所不同。然而，两者都是小型二十面体病毒，在宿主脉管系统中进行系统移动都需要CP。在此，我们表明TRoV CP基因能够促进石竹病毒属病毒的脉管移动。用TRoV CP基因替换RCNMV CP基因可使产生的嵌合病毒移动到未接种的叶片。缺少CP基因或含有不可翻译的TRoV CP基因的RCNMV不会进行系统移动。本报告介绍了TRoV的分子特征，并描述了通过植物病毒CP基因的基因间完全替换对系统移动功能进行的前所未有的互补作用。
