结核分枝杆菌同基因实验室菌株中直接可变重复序列缺失导致的寡核苷酸分型图谱变化
Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain.
作者信息
Aranaz Alicia, Romero Beatriz, Montero Natalia, Alvarez Julio, Bezos Javier, de Juan Lucía, Mateos Ana, Domínguez Lucas
机构信息
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
出版信息
J Clin Microbiol. 2004 Nov;42(11):5388-91. doi: 10.1128/JCM.42.11.5388-5391.2004.
Abstract
Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.
摘要
寡核苷酸分型是结核分枝杆菌复合群生物分子分型的主要工具。出于流行病学目的，只有当菌株的寡核苷酸分型模式相同时，才被认为是克隆性的。我们报告了由一个直接可变重复序列缺失导致的真正同基因菌株（一个临床分离株和一个实验室衍生的传代培养物）寡核苷酸分型谱的变化。如果没有它们之间关系的信息，这些菌株之间的联系就会被忽视。在解释寡核苷酸分型结果和设计数据库时，应考虑进化事件。
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