细菌性眼内炎分子诊断中一个意外的实验陷阱。
An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.
作者信息
Ugahary Luana, van de Sande Wendy, van Meurs Jan C, van Belkum Alex
机构信息
The Rotterdam Eye Hospital, Rotterdam, The Netherlands.
出版信息
J Clin Microbiol. 2004 Nov;42(11):5403-5. doi: 10.1128/JCM.42.11.5403-5405.2004.
Abstract
General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
摘要
眼内炎期间一般引物介导的核糖体DNA扩增可能会提高诊断微生物学的质量。然而，在手术过程中必须格外小心，以免引入污染性细菌DNA。由于存在类似假单胞菌的生物体的DNA，使用消毒碘溶液可能会导致此类污染。
相似文献
1
An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.细菌性眼内炎分子诊断中一个意外的实验陷阱。
J Clin Microbiol. 2004 Nov;42(11):5403-5. doi: 10.1128/JCM.42.11.5403-5405.2004.
2
Effect of povidone-iodine concentration and exposure time on bacteria isolated from endophthalmitis cases.聚维酮碘浓度和暴露时间对眼内炎分离菌的影响。
J Cataract Refract Surg. 2012 Jan;38(1):92-6. doi: 10.1016/j.jcrs.2011.06.030. Epub 2011 Oct 8.
3
Povidone-iodine for endophthalmitis prophylaxis.用于预防眼内炎的聚维酮碘
Am J Ophthalmol. 2014 Mar;157(3):503-4. doi: 10.1016/j.ajo.2013.12.001.
4
Another endophthalmitis treatment.另一种眼内炎治疗方法。
J Cataract Refract Surg. 1998 Nov;24(11):1421-2. doi: 10.1016/s0886-3350(98)80156-9.
5
Reduction of vitreous contamination rate after 25-gauge vitrectomy by surface irrigation with 0.25% povidone-iodine.表面灌洗 0.25%聚维酮碘降低 25G 玻切术后玻璃体污染率。
Retina. 2013 Jan;33(1):143-51. doi: 10.1097/IAE.0b013e318261a6ce.
6
Real-time polymerase chain reaction in the diagnosis of acute postoperative endophthalmitis.实时聚合酶链反应在急性术后眼内炎诊断中的应用。
Am J Ophthalmol. 2012 Jun;153(6):1031-7.e2. doi: 10.1016/j.ajo.2011.12.007. Epub 2012 Mar 3.
7
[Application of molecular method in identification of bacterial endophthalmitis].[分子方法在细菌性眼内炎诊断中的应用]
Zhonghua Yi Xue Za Zhi. 2008 May 27;88(20):1429-32.
8
Diagnosis of bacterial endophthalmitis by broad-range quantitative PCR.广谱定量 PCR 诊断细菌性眼内炎。
Br J Ophthalmol. 2011 Mar;95(3):345-9. doi: 10.1136/bjo.2009.171504. Epub 2010 Jul 31.
9
Effect of operative field irrigation on intraoperative bacterial contamination and postoperative endophthalmitis rates in 25-gauge vitrectomy.25G 玻璃体切割术中手术野冲洗对术中细菌污染和术后眼内炎发生率的影响。
Retina. 2010 Sep;30(8):1242-9. doi: 10.1097/IAE.0b013e3181cea6ab.
10
[Endogenous serratia marcescens endophthalmitis diagnosis with PCR].[采用聚合酶链反应诊断内源性粘质沙雷氏菌眼内炎]
Arch Soc Esp Oftalmol. 2003 May;78(5):281-3.
引用本文的文献
1
Validation of a multi-species-specific PCR panel to diagnose patients with suspected postoperative bacterial endophthalmitis.验证一种多物种特异性 PCR 面板，用于诊断疑似术后细菌性眼内炎的患者。
Acta Ophthalmol. 2022 May;100(3):e827-e832. doi: 10.1111/aos.14964. Epub 2021 Jul 13.
本文引用的文献
1
Reliability of nested polymerase chain reaction in the diagnosis of bacterial endophthalmitis.巢式聚合酶链反应在细菌性眼内炎诊断中的可靠性
Am J Ophthalmol. 2002 Jan;133(1):142-4. doi: 10.1016/s0002-9394(01)01212-0.
2
Molecular diagnosis of microbial contamination in cosmetic and pharmaceutical products: a review.化妆品和药品中微生物污染的分子诊断：综述
J AOAC Int. 2001 May-Jun;84(3):671-5.
3
Use of PCR in endophthalmitis.聚合酶链反应在眼内炎中的应用。
Ocul Immunol Inflamm. 2000 Sep;8(3):189-200.
4
PCR-based evidence of bacterial involvement in eyes with suspected intraocular infection.基于聚合酶链反应的证据表明细菌与疑似眼内感染的眼睛有关。
Invest Ophthalmol Vis Sci. 2000 Oct;41(11):3474-9.
5
Improved detection of microorganisms by polymerase chain reaction in delayed endophthalmitis after cataract surgery.白内障手术后迟发性眼内炎中通过聚合酶链反应改进微生物检测
Ophthalmology. 2000 Jun;107(6):1047-51; discussion 1051-2. doi: 10.1016/s0161-6420(00)00083-x.
6
PCR-RFLP-mediated detection and speciation of bacterial species causing endophthalmitis.聚合酶链反应-限制性片段长度多态性介导的引起眼内炎的细菌种类检测及鉴定
Invest Ophthalmol Vis Sci. 2000 May;41(6):1438-47.
7
Diagnosis of infectious endophthalmitis after cataract surgery by polymerase chain reaction.通过聚合酶链反应诊断白内障手术后感染性眼内炎
J Cataract Refract Surg. 1998 Jun;24(6):821-6. doi: 10.1016/s0886-3350(98)80138-7.
8
Evaluation of microbiological diagnostic techniques in postoperative endophthalmitis in the Endophthalmitis Vitrectomy Study.眼内炎玻璃体切割术研究中术后眼内炎微生物诊断技术的评估
Arch Ophthalmol. 1997 Sep;115(9):1142-50. doi: 10.1001/archopht.1997.01100160312008.
9
Results of the Endophthalmitis Vitrectomy Study. A randomized trial of immediate vitrectomy and of intravenous antibiotics for the treatment of postoperative bacterial endophthalmitis. Endophthalmitis Vitrectomy Study Group.眼内炎玻璃体切除术研究结果。一项关于立即进行玻璃体切除术和静脉注射抗生素治疗术后细菌性眼内炎的随机试验。眼内炎玻璃体切除术研究组。
Arch Ophthalmol. 1995 Dec;113(12):1479-96.
10
Pseudobacteremia attributed to contamination of povidone-iodine with Pseudomonas cepacia.由洋葱伯克霍尔德菌污染聚维酮碘导致的假菌血症。
Ann Intern Med. 1981 Jul;95(1):32-6. doi: 10.7326/0003-4819-95-1-32.
Zotero 插件