Sung Bokyung, Park Seongjoon, Yu Byung Pal, Chung Hae Young
College of Pharmacy, Aging Tissue Bank, Pusan National University, Busan, Korea.
J Gerontol A Biol Sci Med Sci. 2004 Oct;59(10):997-1006. doi: 10.1093/gerona/59.10.b997.
Peroxisome proliferator-activated receptors (PPARs), members of the nuclear hormone receptor superfamily of transcription factors, are key regulators in various pathophysiological processes related to energy metabolism including lipid, carbohydrate metabolism, and inflammation. At present, little information is on the effect of age and calorie restriction (CR) on PPARs. In the present study, we investigated how age and CR (60% of the ad libitum intake) modulate PPARs in kidneys obtained from Fischer 344 rats, ages 13 and 25 months. Results showed that nuclear protein, mRNA level, and DNA binding activity of PPARs decreased with age, while CR blunted the reduction. Our findings were verified in separate experiments in which rats were injected with lipopolysaccharide, with the result of increased susceptibility to inflammation. Based on these findings, we conclude that the altered expression of PPARs may be due to increased oxidative stress with age, and that CR prevents these decreases through its antioxidative action.
过氧化物酶体增殖物激活受体（PPARs）是核激素受体超家族转录因子的成员，是与能量代谢相关的各种病理生理过程的关键调节因子，包括脂质、碳水化合物代谢和炎症。目前，关于年龄和热量限制（CR）对PPARs的影响的信息很少。在本研究中，我们研究了年龄和CR（自由摄入量的60%）如何调节从13个月和25个月大的Fischer 344大鼠获得的肾脏中的PPARs。结果表明，PPARs的核蛋白、mRNA水平和DNA结合活性随年龄增长而降低，而CR减弱了这种降低。我们的发现在用脂多糖注射大鼠的单独实验中得到了验证，结果是对炎症的易感性增加。基于这些发现，我们得出结论，PPARs表达的改变可能是由于年龄增长导致氧化应激增加，而CR通过其抗氧化作用防止了这些降低。
