Park Chan Young, Heo Won Do, Yoo Jae Hyuk, Lee Ju Huck, Kim Min Chul, Chun Hyun Jin, Moon Byeong Cheol, Kim Ihn Hyeong, Park Hyeong Cheol, Choi Man Soo, Ok Hyun Mi, Cheong Mi Sun, Lee Sang Min, Kim Ho Soo, Lee Kon Ho, Lim Chae Oh, Chung Woo Sik, Cho Moo Je
Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Mol Cells. 2004 Oct 31;18(2):207-13.
Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca2+ signals. This diversity results in different physiological responses to particular stimuli. Gm-CaM-4 and -5 are two divergent calmodulin isoforms from the soybean (Glycine max) that have been reported to be involved in plant disease resistance. However, little is known about the pathway by which these specific isoforms transduce the defense signal and up-regulate pathogenesis-related (PR) genes. Here we report that overexpression of GmCaM-4/-5 induces constitutive PR gene expression and enhances disease resistance in wild-type Arabidopsis, but not in the nim1 mutant of Arabidopsis. GmCaM-4/-5 also appear to activate trans-acting elements that bind to cis-acting elements in the Arabidopsis PR-1 promoter. Thus up-regulation of PR genes by these GmCaM isoforms is dependent on NIM1 (Non immunity 1) and unknown transcription factors.
植物产生大量钙调蛋白异构体，这些异构体表现出不同的基因表达模式，并感知不同的Ca2+信号。这种多样性导致对特定刺激产生不同的生理反应。Gm-CaM-4和-5是大豆（Glycine max）中两种不同的钙调蛋白异构体，据报道它们参与植物抗病性。然而，对于这些特定异构体转导防御信号并上调病程相关（PR）基因的途径知之甚少。在此我们报道，GmCaM-4/-5的过表达诱导野生型拟南芥中PR基因的组成型表达并增强抗病性，但在拟南芥的nim1突变体中则不然。GmCaM-4/-5似乎还激活了与拟南芥PR-1启动子中的顺式作用元件结合的反式作用元件。因此，这些GmCaM异构体对PR基因的上调依赖于NIM1（非免疫1）和未知的转录因子。
