Luque I, Herrero A, Flores E, Madueño F
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y CSIC, Facultad de Biología, Spain.
Mol Gen Genet. 1992 Mar;232(1):7-11. doi: 10.1007/BF00299130.
A region of the genome of the cyanobacterium Synechococcus R2, that bears a cluster of genes involved in nitrate assimilation, has been cloned and the relative positions of some of the genes in the region have been determined. Mutations generated by insertion of an antibiotic-resistance gene cassette into the gene encoding nitrite reductase are associated with reduced expression of nitrate reductase; cotranscription of nitrate assimilation genes in the cluster is inferred from this finding.
蓝藻聚球藻R2基因组中一个带有参与硝酸盐同化相关基因簇的区域已被克隆,并且该区域中一些基因的相对位置已被确定。通过将抗生素抗性基因盒插入亚硝酸还原酶编码基因所产生的突变与硝酸还原酶表达降低相关;由此发现推断出该基因簇中硝酸盐同化基因的共转录。