Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library.
作者信息
Kuhlemeier C J, Teeuwsen V J, Janssen M J, van Arkel G A
出版信息
Gene. 1984 Nov;31(1-3):109-16. doi: 10.1016/0378-1119(84)90200-2.
PMID:6098517
Abstract
A strategy for gene cloning in the cyanobacterium Anacystis nidulans R2 was developed which made use of a gene library constructed in a shuttle cosmid vector. The method involved phenotypic complementation of mutants with pooled cosmid DNA. The development of the procedure and its application to the cloning of a third gene involved in nitrate reduction are described.
摘要
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