集胞藻PCC 7942中氮同化调控基因的鉴定与克隆
Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942.
作者信息
Vega-Palas M A, Madueño F, Herrero A, Flores E
机构信息
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-Consejo Superior de Investigaciones Cientificas, Spain.
出版信息
J Bacteriol. 1990 Feb;172(2):643-7. doi: 10.1128/jb.172.2.643-647.1990.
Abstract
Twenty-seven mutants that were unable to assimilate nitrate were isolated from Synechococcus sp. strain PCC 7942. In addition to mutants that lacked nitrate reductase or nitrite reductase, seven pleiotropic mutants impaired in both reductases, glutamine synthetase, and methylammonium transport were also isolated. One of the pleiotropic mutants was complemented by transformation with a cosmid gene bank from wild-type strain PCC 7942. Three complementing cosmids were isolated, and a 3.1-kilobase-pair DNA fragment that was still able to complement the mutant was identified. The regulatory gene that was cloned (ntcA) appeared to be required for full expression of proteins subject to ammonium repression in Synechococcus sp.
摘要
从聚球藻属PCC 7942菌株中分离出27个不能同化硝酸盐的突变体。除了缺乏硝酸还原酶或亚硝酸还原酶的突变体外，还分离出了7个在两种还原酶、谷氨酰胺合成酶和甲基铵转运方面均受损的多效性突变体。其中一个多效性突变体通过用野生型菌株PCC 7942的黏粒基因文库进行转化得到了互补。分离出了三个互补黏粒，并鉴定出一个仍能使该突变体互补的3.1千碱基对的DNA片段。克隆的调控基因（ntcA）似乎是聚球藻属中受铵抑制的蛋白质充分表达所必需的。
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