Huang Yuan-Pin, Lee Hsin-Chun, Cheng Ming-Chu, Wang Ching-Ho
Department of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan.
Avian Dis. 2004 Sep;48(3):581-9. doi: 10.1637/7186-033004R.
The disease caused by infectious bronchitis virus (IBV) produces great economic for the poultry industry. The purpose of this study is to investigate the molecular epidemiology of IBV in Taiwan. An old IBV strain isolated in 1964 and another 31 strains isolated from 1991 to 2003 were selected for N-terminal S1 gene analysis. Based on their phylogenetic tree, 13 strains were selected for sequencing the entire S1 and partial nucleocapsid (N) genes. The results indicated that Taiwanese IBV strains could be divided into two distinct lineages, Taiwan Group I and Taiwan Group II, with one Massachusetts strain and one Chinese strain. No recombination was found between H120 and the Taiwanese strains in the S1 gene. However, the S1 gene showed a noticeably higher divergence than the N gene. The phylogenetic trees constructed from the S1 and N genes indicate that intergenic recombination has occurred. Since most local strains are in Taiwanese clusters, developing vaccines from local strains is necessary for IBV control in Taiwan.
传染性支气管炎病毒（IBV）引起的疾病给家禽业造成了巨大的经济损失。本研究的目的是调查台湾地区IBV的分子流行病学。选取了1964年分离的一株旧IBV毒株以及1991年至2003年分离的另外31株毒株进行N端S1基因分析。根据它们的系统发育树，选取13株进行整个S1基因和部分核衣壳（N）基因的测序。结果表明，台湾地区的IBV毒株可分为两个不同的谱系，即台湾I组和台湾II组，其中包括一株马萨诸塞州毒株和一株中国毒株。在S1基因中未发现H120与台湾地区毒株之间的重组。然而，S1基因的差异明显高于N基因。由S1和N基因构建的系统发育树表明发生了基因间重组。由于大多数本地毒株属于台湾地区的聚类，因此利用本地毒株开发疫苗对于台湾地区控制IBV是必要的。
