Mansuy Virginie, Boireau Wilfrid, Fraichard Annick, Schlick Jean-Luc, Jouvenot Michèle, Delage-Mourroux Régis
Equipe Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, Université de Franche-Comté, U.F.R. Sciences et Techniques, IFR 133, 16 route de Gray, 25030 Besançon Cedex, France.
Biochem Biophys Res Commun. 2004 Dec 10;325(2):639-48. doi: 10.1016/j.bbrc.2004.10.072.
We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA(A) receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA(A) receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA(A) receptor.
我们之前在子宫细胞中鉴定出一种名为gec1的新型雌激素调节基因。GEC1与GABARAP有87%的同源性，而GABARAP是迄今为止发现的唯一一种与微管蛋白和GABA(A)受体相关的蛋白质。然后我们证明了GEC1在体外与微管蛋白和GABA(A)受体相互作用，并促进微管蛋白组装和微管成束。由于所有多克隆抗体都无法区分GEC1和GABARAP这两种蛋白质，因此使用了GEC1-GFP融合蛋白来特异性定位GEC1。GEC1-GFP以分散的模式分布在核周囊泡的细胞质中。总体而言，我们的数据表明GEC1可能是参与GABA(A)受体转运的GABARAP家族的新成员。
