Rolland-Lagan Anne-Gaëlle, Coen Enrico, Impey Stephen J, Bangham J Andrew
John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK.
J Theor Biol. 2005 Jan 21;232(2):157-77. doi: 10.1016/j.jtbi.2004.04.045.
We describe a method for estimating growth parameters in various regions of a developing organ undergoing cell divisions, along with the corresponding changes in organ shape. Growth parameters are computed by coupling clonal analysis with a growth model, allowing a wide range of developmental stages to be covered. The method was applied to the development of dorsal petal lobes of Antirrhinum majus. The resulting description of growth patterns and shape changes is consistent with direct observations using scanning electron microscopy. This method can potentially be applied to other organs, and opens the way to comparative studies of growth and gene expression patterns.
我们描述了一种方法，用于估计正在进行细胞分裂的发育中器官各个区域的生长参数，以及器官形状的相应变化。通过将克隆分析与生长模型相结合来计算生长参数，从而能够涵盖广泛的发育阶段。该方法应用于金鱼草背花瓣叶的发育研究。所得出的生长模式和形状变化描述与使用扫描电子显微镜的直接观察结果一致。此方法有可能应用于其他器官，并为生长和基因表达模式的比较研究开辟了道路。
