Frauenschuh Achim, DeVico Anthony L, Lim Siew Pheng, Gallo Robert C, Garzino-Demo Alfredo
Division of Basic Science, Institute of Human Virology, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA.
Vaccine. 2004 Dec 9;23(4):546-54. doi: 10.1016/j.vaccine.2004.06.024.
Chemokines are key players in the elicitation of immune response, by selectively attracting subpopulations of immune cells to the site of antigen presentation. Therefore, they are natural candidates for modulating immune responses to antigens qualitatively and quantitatively. We have selected chemokines associated with different arms of the immune response, i.e. RANTES/CCL5, B-lymphocyte chemoattractant/CXCL13, and monocyte chemoattractant protein-1/CCL2, and co-injected DNA expression constructs encoding these chemokines with constructs encoding two HIV antigens, gp120 and gp160, in mice. We subsequently measured markers of both cellular and humoral immune responses, and found that these chemokines qualitatively influenced the outcome of immune responses to both antigens, essentially according to their predicted association to Th profiles. These results are relevant towards the engineering of novel vaccine and immune-based therapies, and point to chemokines as candidate adjuvant and immunomodulatory molecules.
趋化因子是引发免疫反应的关键因素，通过选择性地将免疫细胞亚群吸引到抗原呈递部位。因此，它们是在质量和数量上调节对抗原免疫反应的天然候选物。我们选择了与免疫反应不同分支相关的趋化因子，即调节激活正常T细胞表达和分泌因子/CCL5、B淋巴细胞趋化因子/CXCL13和单核细胞趋化蛋白-1/CCL2，并在小鼠中将编码这些趋化因子的DNA表达构建体与编码两种HIV抗原gp120和gp160的构建体共同注射。我们随后测量了细胞免疫反应和体液免疫反应的标志物，发现这些趋化因子基本上根据它们与Th谱的预测关联，在质量上影响了对抗原的免疫反应结果。这些结果与新型疫苗和基于免疫的疗法的工程相关，并指出趋化因子作为候选佐剂和免疫调节分子。
