Jordan Dianna M, Knittel Jeffrey P, Schwartz Kent J, Roof Michael B, Hoffman Lorraine J
Iowa State University, Veterinary Diagnostics and Production Animal Medicine, Ames, IA 50010, USA.
Vet Microbiol. 2004 Nov 30;104(1-2):83-90. doi: 10.1016/j.vetmic.2004.09.004.
Transmission of Lawsonia intracellularis from experimentally inoculated pigs to naive swine was demonstrated in this study. The study was conducted using conventional pigs divided into three groups as follows: principles inoculated with L. intracellularis, sentinels, and controls. The pigs were inoculated and paired on 13 and 9 days post-inoculation with a sentinel pig for 7 days. Fecal samples and serum samples were collected throughout the study for polymerase chain reaction (PCR) and antibody testing by indirect fluorescent antibody techniques. After co-mingling, the inoculated group was necropsied; sentinel and control pigs were necropsied 7-14 days later. The intestinal tracts were evaluated grossly and microscopically for lesions. PCR was performed on intestinal mucosal scrapings and feces. Warthin-Starry and fluorescent antibody staining procedures were conducted to confirm colonization with L. intracellularis. Gross and microscopic lesions typical of porcine proliferative enteropathy (PPE) were observed in both the inoculated and sentinel groups. Transmission was demonstrated from inoculated principle pigs to sentinel pigs. PCR results detected cyclical shedding of L. intracellularis in the feces. Seroconversion occurred in pigs that were exposed to L. intracellularis. From this study, it was demonstrated that transmission of L. intracellularis can occur easily in an environment with experimentally infected pigs and that PCR can be a useful tool to monitor fecal shedding of the organism.
本研究证实了胞内劳森菌可从经实验接种的猪传播至未感染的猪。该研究使用常规猪分为以下三组进行：接种胞内劳森菌的原发病例组、哨兵猪组和对照组。在接种后第13天和第9天对猪进行接种，并与一头哨兵猪配对7天。在整个研究过程中收集粪便样本和血清样本，用于聚合酶链反应（PCR）检测以及通过间接荧光抗体技术进行抗体检测。混群后，对接种组猪进行剖检；7 - 14天后对哨兵猪和对照组猪进行剖检。对肠道进行大体和显微镜检查以评估病变情况。对肠道黏膜刮片和粪便进行PCR检测。采用Warthin - Starry染色和荧光抗体染色程序以确认胞内劳森菌的定植情况。在接种组和哨兵猪组均观察到典型的猪增生性肠炎（PPE）的大体和显微镜病变。证实了胞内劳森菌可从接种的原发病例猪传播至哨兵猪。PCR结果检测到胞内劳森菌在粪便中的周期性排出。接触胞内劳森菌的猪出现了血清学转化。从本研究可知，胞内劳森菌在有实验感染猪的环境中容易发生传播，并且PCR可作为监测该菌粪便排出情况的有用工具。
